Research Paper Volume 12, Issue 7 pp 5625—5639

LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p

Figure 2. LncRNA 2310043L19Rik overexpression inhibits myoblast differentiation. (A) Real-time PCR analysis of 2310043L19Rik expression in C2C12 transfected with control pcDNA3.1 or lnc-231. (B) Representative photographs of MyHC immunofluorescence staining in C2C12 cells differentiated for 4 d showing that lnc-231 inhibited myoblast differentiation. Positively stained cells were quantified (C). (D) Real-time PCR analysis of MyHC expression in C2C12 transfected with control pcDNA3.1 or lnc-231 then cultured in DM for 4 days. (E) Representative photographs of MyoG immunofluorescence staining in C2C12 cells differentiated for 4 d showing that lnc-231 inhibited myoblast differentiation. Positively stained cells were quantified (F). (G) Western blot results showing that 2310043L19Rik overexpression significantly decreased the protein expression levels of MyHC in C2C12 cells differentiated for 3 d, and relative protein lever was performed.