**Figure 2.** **LINC01198 enhanced proliferation and invasion of glioma cells.** (**A**) Basal level of LINC01198 was detected using qRT-PCR in the panel of glioma cell lines we enrolled and human normal glial cell line HEB, acted as normal control. qRT-PCR was actually performed independently three times (n=3). Shown was the stable result we achieved. Multiple comparisons were made using one way ANOVA analysis (Bonferroni approach). *** P<0.001 in comparison with HEB group. (**B**) Confirmation of LINC01198 variation by qRT-PCR after endogenous LINC01198 was being artificially manipulated using shRNA (T87G) or over-expression (Hs 683) strategy. The experiment was done independently four times; Shown was the stable result we achieved. Two-tailed, Mann-Whitney U test was employed to analyze the statistical difference (Mann-Whitney U=0, P=0.0286). (**C**) Proliferative variation of glioma cells was monitored by MTT approach after LINC01198 was being stably knocked down (T87G) or over-expressed (Hs 683). The experiment was carried out independently three times, shown was the representative one. Independent sample T-test was applied to analyze the proliferative difference, * P<0.05, ** P<0.01 compared with control group. (**D**) Clonogenesis assay was used to verify the clonogenic variations of glioma cells after LINC01198 was stably knocked down (T87G) or re-expressed (Hs 683). Two-tailed, unpaired T-test was used to analyze the colony formation difference (T87G, t=12.14, df=4, P=0.0003; Hs 683, t=10.14, df=4, P=0.0005); (**E**) Transwell assay was applied to analyze the invasive variation of glioma cells after LINC01198 was being stably knocked down (T87G) or over-expressed (Hs 683). Two-tailed, unpaired T-test was used to analyze the statistical difference (T87G, t=17.20, df=4, P<0.001; Hs 683, t=11.12, df=4, P<0.001). *** P<0.001 relative to control group. (**F**) Subcutaneous xenograft nude mice model was used to verify the proliferative variation of glioma cells whose LINC01198 was being stably knocked down (T87G) or over-expressed (Hs 683). Two tailed, unpaired T-test was used to analyze the significant difference (T87G, t=9.456, df=12, P<0.0001; Hs 683, t=4.003, df=4, P=0.0161).