Research Paper Volume 12, Issue 7 pp 5675—5692

STAT5A induced LINC01198 promotes proliferation of glioma cells through stabilizing DGCR8

Figure 2. LINC01198 enhanced proliferation and invasion of glioma cells. (A) Basal level of LINC01198 was detected using qRT-PCR in the panel of glioma cell lines we enrolled and human normal glial cell line HEB, acted as normal control. qRT-PCR was actually performed independently three times (n=3). Shown was the stable result we achieved. Multiple comparisons were made using one way ANOVA analysis (Bonferroni approach). *** P<0.001 in comparison with HEB group. (B) Confirmation of LINC01198 variation by qRT-PCR after endogenous LINC01198 was being artificially manipulated using shRNA (T87G) or over-expression (Hs 683) strategy. The experiment was done independently four times; Shown was the stable result we achieved. Two-tailed, Mann-Whitney U test was employed to analyze the statistical difference (Mann-Whitney U=0, P=0.0286). (C) Proliferative variation of glioma cells was monitored by MTT approach after LINC01198 was being stably knocked down (T87G) or over-expressed (Hs 683). The experiment was carried out independently three times, shown was the representative one. Independent sample T-test was applied to analyze the proliferative difference, * P<0.05, ** P<0.01 compared with control group. (D) Clonogenesis assay was used to verify the clonogenic variations of glioma cells after LINC01198 was stably knocked down (T87G) or re-expressed (Hs 683). Two-tailed, unpaired T-test was used to analyze the colony formation difference (T87G, t=12.14, df=4, P=0.0003; Hs 683, t=10.14, df=4, P=0.0005); (E) Transwell assay was applied to analyze the invasive variation of glioma cells after LINC01198 was being stably knocked down (T87G) or over-expressed (Hs 683). Two-tailed, unpaired T-test was used to analyze the statistical difference (T87G, t=17.20, df=4, P<0.001; Hs 683, t=11.12, df=4, P<0.001). *** P<0.001 relative to control group. (F) Subcutaneous xenograft nude mice model was used to verify the proliferative variation of glioma cells whose LINC01198 was being stably knocked down (T87G) or over-expressed (Hs 683). Two tailed, unpaired T-test was used to analyze the significant difference (T87G, t=9.456, df=12, P<0.0001; Hs 683, t=4.003, df=4, P=0.0161).