Research Paper Volume 12, Issue 7 pp 5693—5715

Indian Hedgehog regulates senescence in bone marrow-derived mesenchymal stem cell through modulation of ROS/mTOR/4EBP1, p70S6K1/2 pathway

Figure 5. IHH alleviated senescence and induced proper differentiation in BMSC. (A) Senescent BMSC (n=5) incubated with and without IHH for 24 hrs, then stained by SA-β-gal stain. (B) Non-senescent and senescent BMSC (n = 5) were incubated in osteogenic differentiation medium with and without IHH for 21 days. Osteogenesis measured using Alizarine-Red-S stain. (C) Non-senescent and senescent BMSC (n = 5) were incubated in adipogenesis differentiation medium with and without IHH for 21 days. Adipogenesis measured by staining fat droplets using Oil-Red-O stain. All data obtained are indicated as mean ± SEM. **P < 0.01, ***p <0.001, ****p <0.0001.