Figure 2. AMPK KO protects OHCs from damages and losses. (A) Representative immunofluorescent surface preparation images of OHCs from four genotype mice aged 10-12 months were captured at the frequency-specific regions (8, 11.3, 16, and 32 kHz) of the cochleae. The OC was dissected for the staining of hair cells with Myosin7a (green). Scale bar=20 μm. (B) Quantification of OHCs survivals in four genotype mice aged 10-12 months. AMPK knockouts increased the number of surviving OHCs in cochlea, differed significantly between Tg-B1 (red bars) and AMPK+/−/Tg-B1 (blue bars) mice at 8, 11.3, 16 and 22.6 kHz regions. Values are presented as mean ± SEM and evaluated with two-way ANOVA followed by Bonferroni post-test. (* P<0.05, ** P<0.01, ***P<0.001, **** P<0.0001; n=7 or 8). (C) Representative confocal microscopy images from four genotypes of the cochleae. For reference, frequency regions of interest were indicated by the arrowheads. Scale bar=50 μm.