Priority Research Paper Volume 12, Issue 7 pp 5590—5611

Down-regulation of AMPK signaling pathway rescues hearing loss in TFB1 transgenic mice and delays age-related hearing loss

Figure 5. AMPK KO rescues Endocochlear Potential (EP) in Tg-B1 mice. (A) Profound EP loss in 10-12 months Tg-B1 (red bars) mice was observed as compared to all three other genotypes (Tg-B1 vs. WT, p=0.0023; Tg-B1 vs. AMPK+/−/Tg-B1, p=0.0023; Tg-B1 vs. AMPK+/−, p=0.0005; one-way ANOVA followed by Bonferroni post-test). * P<0.05, ** P<0.01, ***P<0.001. (B) Representative cochlear cross-sections stained with H&E showed gross morphology of stria vascularis in the apical, middle and basal turns of cochleae from four genotypes of mice at 10-12 months. No visible atrophy was found. Scale bar=50 μm. (C) The histograms show no significant difference in averaged sectional area of stria vascularis for all four aged genotypes (n=6 for each group; F(3,20)=0.8244, p=0.4958, two-way ANOVA followed by Bonferroni post-test).