Figure 4. Kl markedly ameliorates pulmonary fibrosis in an ex vivo model. Mouse PCLSs that were pre-incubated with vehicle (Vel) or mouse rKL for 24 h were randomized to be treated with control cocktail (CC) or fibrosis cocktail (FC) with or without KL for another 48 h. mRNA levels of Acta2, Fn1, Col1a1, Ctgf, Tnc, and Serpine1 were assessed by qPCR (A), protein levels of fibronectin and α-SMA were measured by western blotting (B), and fibronectin, α-SMA, and collagen I were stained by immunofluorescence staining (C) in the mouse PCLSs from each group (CC+Veh, white bars; FC+Veh, light gray bars; CC+rKL, dark gray bars; FC+rKL, black bars). Scale bars = 50 μm. **P < 0.01 vs. CC+Veh. #P < 0.05 or ##P < 0.01 vs. FC+Veh.