Figure 6. Autophagy flux in skeletal muscle of young WT and old WT mice. (A) Western blot images. (B–E) Statistical graphs. Autophagy flux was calculated by the fold of changes in the expression of LC3b-II, P62, Bnip3, and Lamp-1 induced by colchicine. Data represent mean ± SE, n=3. *statistically significant.