Research Paper Volume 12, Issue 7 pp 5992—6017

PCNA-associated factor KIAA0101 transcriptionally induced by ELK1 controls cell proliferation and apoptosis in nasopharyngeal carcinoma: an integrated bioinformatics and experimental study

Figure 5. Cell cycle and DNA synthesis process suppressed after downregulation of KIAA0101. (A) LinkedOmics analysis of positively and negatively related genes with KIAA0101 in TCGA HNSC samples. (B) Metascape color clustering of positively correlated significant genes, P<0.0001, FDR(BH)<0.0001, Statistic>0). Each color box represents a biological process or pathway. (C) LinkeOmics GSEA KEGG module analysis of differentially expressed genes for associations between KIAA0101 expressions and Hiseq RNA expression profiles in TCGA HNSC samples revealed that cell cycle was positively enriched. (D) S and G2/M phases of shKIAA0101 groups were decreased compared with those in shCtrl group by flowcytometry. Histogram is the average ratio (mean ± SD) of three independent experiments. *P<0.005. (E) LinkeOmics GSEA KEGG analysis revealed that DNA replication was also identified with the strongest association with KIAA0101-higher expression. (F) BrdU incorporation in cultured CNE-2Z cells following control (shCtrl) and shKIAA0101 lentivirus infection. Histogram is the average ratio (mean ± SD) of three independent experiments. ****p<0.00001 by Student’s t test.