Research Paper Volume 12, Issue 8 pp 6586—6599

The expression of PDGF-BB predicts curative effect in locally advanced esophageal squamous cell carcinoma treated by radiotherapy

Figure 3. Depletion of PDGFB inhibits esophageal squamous cell carcinoma cell growth and induces apoptosis. Western blotting analysis showed that PDGFB expression was down-regulated after lentiviral transduction (A). Depletion of PDGFB resulted in a lower proliferation rate in KYSE30 and KYSE150 cells as determined by the Cell Counting Kit-8 (B) and colony formation assays (C). In addition, the transwell assay was applied to show that PDGFB depletion also reduced cell invasion (D). Depletion of PDGFB could also promote apoptosis of both cell-lines (E). **P < 0.01, ***P < 0.001.