Research Paper Volume 12, Issue 8 pp 6586—6599

The expression of PDGF-BB predicts curative effect in locally advanced esophageal squamous cell carcinoma treated by radiotherapy

Figure 4. Inhibition of PDGFB enhanced the sensitivity of esophageal squamous cell carcinoma cells to ionizing radiation (IR), a treatment modality that suppressed PDGF-BB-induced migration, and did so by blocking the PI3K/AKT pathway. PDGFB-knockdown in cancer cells showed lower colony formation capacity than control cells after IR (A). In addition, results of the transwell assay suggested that PDGF-BB can promote KYSE30 and KYSE150 cell migration while IR can suppress it (B). Western blot analysis showed that the PI3K/AKT pathway was activated by PDGF-BB, and pretreatment with IR suppressed PDGF-BB-induced phosphorylation of PI3K and AKT in both cell-lines (C). *P < 0.05, **P < 0.01, ***P < 0.001.