Research Paper Volume 12, Issue 7 pp 6030—6036

Elevated p38MAPK activity promotes neural stem cell aging

Figure 2. p38MAPK activity regulates NSC/progenitor aging in vitro. (A) Quantification of neurospheres from young (2 month-old) and aged (over 24 month-old) C57BL/6J mice (n=3). (B) P-p38MAPK, SOX2 and p16Ink4a expression in neurospheres derived from animals at the indicated ages (n=3). (C) Analysis of MAPK isoforms in neurospheres. (D) Representative image and (E) quantification of neurospheres derived from the SVZ of young and aged C57BL/6J mice treated with p38MAPK inhibitor (PH-797804) or control (DMSO) (n=4). (F) Quantification of the diameter of secondary neurospheres derived from aged mice treated with PH-797804 or control (n=4). (G) Representative western blot of P-p38MAPK, p38MAPK, SOX9 and ß-actin in 2ry neurospheres from aged mice (n=2). (H) Quantification of Sox2 mRNA levels in aged cells (n=3).