Figure 3. Pharmacological inhibition of p38MAPK rejuvenates NSC function in vitro. (A) Representative image and (B, C) quantification of number of neurospheres and their diameter at passage 2 (2ry) and 7 (7ry) from SVZ C57BL/6J mice (n=3). (D) Representative western blot of P-p38MAPK, SOX2, p16Ink4a and ß-actin at indicated conditions (n=3). (E) Relative number of neurospheres formed from passage 7 cells treated with p38MAPK inhibitor (PH-797804) or control (DMSO) (n=3). (F) Quantification of the diameter of neurospheres from 7ry passage treated with PH-797804 or control (n=3). (G) Sox2, Sox9 and Mkp1 expression in neurospheres maintained for passages (n=3).