Figure 2. Aspirin reduces expression of profibrogenic associated mediators and TLR4. (A–C) mRNA expression of early markers of fibrogenesis including α-SMA, collagen-a1 and TGF-β1 was detected by real-time PCR. (D) Western blot was employed to detect the expression ofα-SMA, collagen-a1 and TGF-β1 in liver tissues. (E, F) TLR4 expression was tested by real-time PCR and immunochemistry analysis. (×200; scale bar: 100μm) *P<0.05, **P<0.01.