Research Paper Volume 12, Issue 8 pp 6630—6643

γ-Mangostin isolated from Garcinia mangostana L. suppresses inflammation and alleviates symptoms of osteoarthritis via modulating miR-124-3p/IL-6/NF-κB signaling

Figure 3. γ-MS treatment improved the mobility in OA mice and downregulated the expression of inflammatory markers. (A) Total distance travelled (during the test period) comparison of OA control; MS and γ-MS treated OA mice, significant recovery of OA is observed γ-MS treated mice in comparison to control and MS treatment group; (B) Significant decrease in expression of IL-6, TNF-α, INF-y and NFkB were observed in y-MS treatment group in comparison to the control group; (C) Western blot and bands quantified using densitometry analysis (ImageJ software). The treatment of γ-MS resulted in a markedly decrease in the expression of TNF-α, NFkB, STAT3, and COX2 as compared to the control group. The experiments were performed three times independently. Right panel represents the quantitative representation of 3 independent Western blot analyses. *P<0.05, **P<0.01 and ***P<0.001.