Research Paper Volume 12, Issue 8 pp 6630—6643

γ-Mangostin isolated from Garcinia mangostana L. suppresses inflammation and alleviates symptoms of osteoarthritis via modulating miR-124-3p/IL-6/NF-κB signaling

Figure 4. γ-MS mediated effects were associated with increased miR-124-3p. (A) qPCR analysis of different inflammation-associated miRs. SW982 cells were stimulated with IL-6 and showed a significantly decreased level of miR-24, miR-98-5p and miR-124-3p (the most responsive miR) while γ-MS treatment significantly increased these miRs. (B) Mir-Target prediction shows the 3’UTR sites of IL-6 and NF-κB respectively targeted by miR-98-5p and miR-124-3p. (C, D) qPCR and Western blot analysis (quantified band by densitometry analysis using ImageJ software) demonstrated that inhibitor of miR-124-3p led to an elevated level of inflammatory markers, TNF-α, IFN- γ, IL-6 and MMP7 while the mimic molecule reduced the level. *P<0.05, **P<0.01 and ***P<0.001.