Figure 1. Expression of Sox2OT-V7 in tissue samples and cell lines and its correlation with autophagy markers. (A) Sox2OT-V7 expression in four OS cell lines and a normal osteoblast cell line was determined by qPCR. (B) Sox2OT-V7 expression in 32 paired OS and nontumorous tissue samples was determined by qPCR. (C, D) Sox2OT-V7, and Beclin 1 expression in 20 chemosensitive and 12 chemoresistant OS tissues was determined by qPCR. (E) The correlation Sox2OT-V7 and Beclin 1 mRNA expression in tissue samples was analyzed by Pearson’s correlation analysis. (F) The protein content and localization of Beclin 1 and LC3II in tissue samples was revealed by IHC staining. The data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01.