Figure 3. LncRNA Sox2OT-V7 silencing attenuates Dox-induced autophagy in OS cells. (A–D) Sox2OT-V7 silencing in U2OS cells was achieved by infection with Lsh-Sox2OT-V7. Sox2OT-V7-silenced U2OS cells were treated with Dox, and examined for the protein levels of LC3II, Beclin 1, and p62 were examined. (E) Sox2OT-V7-silenced U2OS cells with stable eGFP-LC3 expression were treated with Dox (5 μM) for 24 h, and the formation of puncta was examined by using a confocal microscope. Representative images are presented. (F–I) Sox2OT-V7-silenced U2OS cells were cotreated with Dox (5 μM) and 3-MA (5 μM) for 24 h, and he protein levels of LC3 II, Beclin 1, and p62 were examined. The data are presented as the mean ± SD of three independent experiments. *P<0.05, **P<0.01, compared to the control group; #P<0.05, ##P<0.01, compared to the Dox group.