Figure 3. Detection of recurrence-associated lncRNAs in serum exosomes. (A) Electron microscopy images of exosomes. (B) Nanoparticle tracking analysis of the size distributions of exosomes. (C) Western blotting analysis of the markers of exosomes (CD81, CD63, ALIX and TSG101). (D–L) Correlation analyses between each lncRNA expression in tissues and in matched serum exosomes; Red and blue dots represent the lncRNA levels in recurrence and nonrecurrence CRC patients of the discovery cohort, respectively; [Spearman test].