Figure 2. Downregulation of FOXK1 inhibits metastasis in vivo. (A) Lung tissues were obtained from nude mice injected with differently treated MGC803 cells (n=5). (B) The number of lung nodules per nude mouse was determined. (C) The lung weight of each nude mouse was calculated. (D, E) Representative images of H&E staining of lung sections (scale bar, 100 μm and 50 μm) and IHC staining of FOXK1, LC3-II, P62, E-cadherin and MMP9 levels in lung tissue (scale bar, 100 μm and 50 μm). (F) MGC803 cells transfected with LV-shFOXK1-1 and LV-shFOXK1-2 were orthotopically transplanted into the stomach walls of nude mice (n=5). (G, H) Weight of gastric wall tumors (g) and number of metastatic tumors (H) at 28 days post transplantation with MGC803 cells. (I, J) Representative images (I) and number (J) of diffuse liver metastases. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.