Research Paper Volume 12, Issue 7 pp 6129—6150

Inhibiting Forkhead box K1 induces autophagy to reverse epithelial-mesenchymal transition and metastasis in gastric cancer by regulating Myc-associated zinc finger protein in an acidic microenvironment

Figure 5. MAZ is a new transcription activation target of FOXK1. (A) Analysis of the 9969 overlapping FOXK1 loci for overrepresented sequence elements identified significantly enriched FOXK1-like DNA-binding motifs, and the results showed that 12.92% of the FOXK1 genomic loci contained at least one of the motifs. (B) Potential binding sites for FOXK1 at the 5'-UTR of MAZ. (C) FOXK1 and MAZ coexpression in the TCGA GC dataset was analyzed by querying the open database ChIPBase v2.0 (r: 0.3449, p = 5.1e-14). The mRNA (D) and protein (E) levels of MAZ in MGC803 and AGS cells were attenuated by the silencing of FOXK1. The data are presented as the means ± S.D.s from three independent experiments. ** P < 0.01, *** P < 0.001, and **** P < 0.0001.