Research Paper Volume 12, Issue 7 pp 6172—6190

USF1-mediated upregulation of lncRNA GAS6-AS2 facilitates osteosarcoma progression through miR-934/BCAT1 axis

Figure 3. GAS6-AS2 knockdown impaired the proliferation and promoted cell apoptosis. (A) qPCR detected GAS6-AS2 levels in MG63 and 143B cells after transfection with GAS6-AS2 siRNAs. (B) CCK-8 assays. (C and D) EdU assays. (E) Clonogenic assays evaluated the effects of GAS6-AS2 knockdown on cell colony formation capabilities in MG63 and 143B cells. (F) Flow cytometry analyses determined the cell apoptosis. (G) Western blot assays detected caspase 3/9 protein expression. * P < 0.05, **P < 0.01.