Research Paper Volume 12, Issue 7 pp 6172—6190

USF1-mediated upregulation of lncRNA GAS6-AS2 facilitates osteosarcoma progression through miR-934/BCAT1 axis

Figure 4. Knockdown of GAS6-AS2 suppressed OS tumorigenesis in vivo. (A) qPCR detected the expression of GAS6-AS2 in OS cells after infecting with GAS6-AS2 shRNA lentivirus. (B) The stable lncRNA GAS6-AS2 knockdown MG63 cells were used for in vivo tumorigenesis assays. The tumor volumes were recorded every 5 days, and the tumor volume-time curves were shown. (C) The photograph and comparison of excised tumor sizes in MG63 cells. (D) The tumor weights were analyzed. * P < 0.05, **P < 0.01.