Research Paper Volume 12, Issue 7 pp 6172—6190

USF1-mediated upregulation of lncRNA GAS6-AS2 facilitates osteosarcoma progression through miR-934/BCAT1 axis

Figure 7. BCAT1 was targeted by miR-934 in OS cells. (A) Heatmap of differentially expressed genes in GSE36001. (B) Volcano map. (C) Venn diagram showed the intersection of up-regulated genes in GSE36001 dataset, “miRDB” predicted miR-934 target genes and “starbase” predicted miR-934 target genes. (D) Relative BCAT1 expression in GSE36001 dataset. (E) Relative BCAT1 expression in 157 OS samples. (F) qPCR analyses detected the relative BCAT1 levels. (G) CCK-8 assays. (H) Clonogenic assays. (I) Binding sites between miR-934 and 3’UTR of BCAT1 mRNA. (J) Luciferase activity detection. (K) Real-time PCR analyses measured relative BCAT1 levels. * P < 0.05, **P < 0.01.