Research Paper Volume 12, Issue 7 pp 6172—6190

USF1-mediated upregulation of lncRNA GAS6-AS2 facilitates osteosarcoma progression through miR-934/BCAT1 axis

Figure 8. GAS6-AS2 modulated OS cell malignancies via miR-934/BCAT1 in OS cells. (A) GAS6-AS2 and BCAT1 expressing levels in OS cells were assessed by qPCR analyses. (B) Real-time PCR analyzed BCAT1 expressing levels in OS cells after GAS6-AS2 was overexpressed or silenced. (C) Real-time PCR determined BCAT1 expressing levels after the upregulation of GAS6-AS2 or overexpression of miR-934. (D) Western blot measured BCAT1 proteins. (E) CCK-8 assays. (F) Wound-healing assays. * P < 0.05, **P < 0.01.