Research Paper Volume 12, Issue 7 pp 6191—6205

Long non-coding RNA TPT1-AS1 promotes angiogenesis and metastasis of colorectal cancer through TPT1-AS1/NF90/VEGFA signaling pathway

Figure 2. TPT1-AS1 promotes CRC cells invasion and metastasis. Transwell assays were performed to detect the migrative and invasive ability of sh-control or TPT1-AS1-knockdown HCT116 cells (A) and control or TPT1-AS1-overexpressed SW480 cells (B). (C) Liver metastasis mice models were used to detect the metastatic ability of HCT116 cells transfected with sh-NC or sh-TPT1-AS1. (D) The liver metastatic nodes of indicated mice were counted and HE staining was performed to confirm the tissue type. *P < 0.05, **P < 0.01, ***P < 0.001.