Figure 4. Impact of RMF on lymphocyte homing to peripheral lymphoid tissues. (A) Demonstrative pictures of the spleen and the mass of the spleen was weighed and the mass of body weight (BW) was calculated. (B) Proportions of CD4+ as well as CD8+ T lymphocytes in the spleen and lymph nodes were determined by flow cytometry. The overall cell numbers as well as CD4+ and CD8+ cell numbers in the (C) spleens and (D) lymph nodes. (E) Spinal cords from the treated mice at day 20 following vaccination were stained with an antibody specific for CD4. Scale bar, 50 μm. Data are shown as the mean ± SEM of three independent experiments. *P <0.05, **P <0.01, ***P <0.001.