Figure 8. Effect of RMF on spinal cord. Spinal cords from the treated mice at day 20 after immunization were stained with an antibody specific for (A) IFN-γ, (B) IL-2, and (C) IL-17. Scale bar, 50 μm. (D) The diagram presents the proportion of positive cells in EAE lesions or corresponding areas (control, n = 3; RMF, n = 3; EAE, n = 6; EAE+RMF, n = 6). (E) Heatmap of IFN-γ and IL-17 expression difference in the spinal cord. (F) The expression level of CD4, IFN-γ and IL-17 mRNA in spinal cord was measured by qPCR. Data are shown as the mean ± SEM of three independent experiments. *P <0.05, **P <0.01, ***P <0.001.