Figure 2. Distribution of uc.333 in various tissues and in hepatocytes. (A) Distribution of uc.333 in various tissues of HFD-fed mice (n = 5). (B) Representative images of FISH detecting endogenous lncRNA uc.333 molecules (green) in HepG2 cells. Nucleus (blue) was stained with DAPI. Scale bar, 25 μm. (C) Cellular fractionation assay in HepG2 was performed using quantitative real-time PCR using a specific cytosol control (tubulin) and a specific nuclear control (gene histone). Data are mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group (Student’s t test).