Figure 5. Interaction between Uc.333 and miR-223. (A) MiRNA-wide expression profiling in the HepG2 cells transfected with si-uc.333 (B) Expression of miR-223, miR-1296, miR-6067, and miR-6889 in the HepG2 cells transfected with si-uc.333 for 24 h. (C) Relative level of miR-223 in si-uc.333-infected HepG2 cells. (D) The sequence of miR-223 and its partial complementarity with uc.333. (E) Levels of miR-223 in the HepG2 cells transfected with Ad-uc.333 WT or Ad-NC mutant for 48 h. (F) Real-time PCR showed that miR-223 was decreased in HepG2 cells induced with IL-6 or TNF-α for 48 h. (G) Level of miR-223 in the livers of mice injected with IL-6 or TNF-α for 7 days. (H) Level of miR-223 in HepG2 cells transfected with miR-223 mimic with or without Ad-uc.333. (I) Tranfection with Ad-uc.333 significantly decreased the mRNA level of FOXO1, but Ad-uc.333-mut did not change the mRNA level of FOXO1 (Figure 5I). Data are mean ± SEM; *P < 0.05; **P < 0.01 vs. control group. (C, E: Student’s t test; B, F, G, H, I: analysis of variance [ANOVA]).