Research Paper Volume 12, Issue 8 pp 6667—6679

Ultraconserved element uc.333 increases insulin sensitivity by binding to miR-223

Figure 6. miR-223 regulated IR through direct targeting of FOXO1. (A) Protein levels of P-AKT, AKT, P-GSK, GSK, and GAPDH in HepG2 cells transfected with mimic miR-223 or NC for 48 h, as analyzed using Western blot. (B) Protein levels of P-AKT, AKT, P-GSK, GSK, and GAPDH in HepG2 cells transfected with miR-223 inhibitor or NC for 48 h, as analyzed using Western blot. (C) Protein levels of P-AKT, AKT, P-GSK, GSK, and GAPDH in HepG2 cells transfected with mimic miR-223 or NC for 48 h, as analyzed using Western blot. Data are mean ± SEM; *P < 0.05; **P < 0.01 vs. control group (Student’s t test).