Research Paper Volume 12, Issue 8 pp 6680—6699

Long intergenic non-protein coding RNA 324 prevents breast cancer progression by modulating miR-10b-5p

Figure 3. Overexpression of LINC00324 attenuates the cancer-related biological characteristics of MDA-MB-231 cells. (A) Lentivirus-mediated LINC00324 overexpression vectors were constructed and validated by qRT-PCR. (B) Proliferation of MDA-MB-231 cells in response to LINC00324 overexpression, measured using MTT assay. (C, D) Representative images of transwell assay showing MDA-MB-231 cells’ migration across the membrane after LINC00324 overexpression. Shown are images of immunohistochemical staining and the scratch wound healing assay. The histograms show the average number of migrated cells per field, calculated from five representative fields. (E) Apoptosis was assessed by flow cytometry in MDA-MB-231 cells after overexpression of LINC00324. All data are shown as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are from three independent experiments (A, B), or are representative of three independent experiments with similar results (CE).