Research Paper Volume 12, Issue 7 pp 6260—6275

Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metformin

Figure 7. Metformin restores cell viability and proliferation in DM1 fibroblasts. (A) Cell viability of DM1 (n=5) and control (n=3) fibroblasts measured in MTT studies after treatment with 1 μM of metformin for 72 h. (B, C) Representative image and quantification of p-H3 (Ser10) staining in the same conditions in controls (n=3) and DM1 (n=7). (D, E) Representative image of Ki-67 staining and quantification in controls (n=2) and DM1 cells (n=3). (F) mRNA levels of BMI-1, p16INK4a and p21CIP in cells treated or not with 1 μM of metformin for 72 h (n≥2). (G) mRNA levels of DMPK and MBNL1 in the same conditions (n=3).