Research Paper Volume 12, Issue 7 pp 6276—6291

DNA N6-methyladenine modification in hypertension

Figure 2. Effect of 6mA DNA level and its modulators in leukocytes and vasculature of mice and rat hypertension models. (A, B) Elevated SBP and diastolic blood pressure (DBP) in angiotensin II (Ang II)-infused C57BL6 wild-type mice (A) and Dahl salt-sensitive rats treated with high salt (HS, 8% NaCl) (B) compared with their controls. (C) Reduced 6mA DNA level in leukocytes of mice and rat hypertension models. (D, E) Representative immunohistochemistry (IHC) and quantification of ALKBH1, N6AMT1 and 6mA levels in vascular smooth muscle cells (VSMCs) of mouse thoracic aorta with sterile saline (Ctrl) or Ang II infusion. Scale bar: 50 μm. (F) Spearman correlation coefficient for m6A level correlated with ALKBH1 or N6AMT1 protein levels in VSMCs of mouse thoracic aorta. (G, H) Representative IHC and quantification of ALKBH1, N6AMT1 and 6mA levels in VSMCs of rat thoracic aortas with low salt (LS; 0.4% NaCl) or HS treatment. Scale bar: 50 μm. (I) Spearman correlation coefficient for m6A level correlated with ALKBH1 or N6AMT1 protein levels in VSMCs of rat thoracic aorta. Data are mean ± SD and were compared by unpaired t test in (AC, E and H).