Figure 4. Knockdown of ALKBH1 suppresses Ang II-induced VSMC phenotype transformation, proliferation and migration. (A, B) Representative western blot and quantification of levels of ALKBH1, alpha-smooth muscle actin (α-SMA), CALPONIN, and osteopontin (OPN) in si-RNA-ALKBH1 (Si-ALKBH1)– or si-RNA Control (Si-CN)–transfected HASMCs with Ang II treatment or not. (C–F) Representative images of EdU staining, HASMC migration distance and number with Si-ALKBH1 and/or Ang II treatment and quantification. Scale bar: 100 μm. *P < 0.05, **P < 0.01, compared with Si-CN and no treatment of Ang II. #P < 0.05 compared with Si-CN and Ang II treatment. Data are mean ± SD (n= 3/group for A and B, n= 5/group for C–F). One-way ANOVA followed by Bonferroni’s multiple comparison test was used for statistical analysis in B and D.