Research Paper Volume 12, Issue 7 pp 6306—6323

Bone marrow mesenchymal stem cells transplantation alleviates brain injury after intracerebral hemorrhage in mice through the Hippo signaling pathway

Figure 2. The astroglial mesenchymal phenotype switching of astrocytes in ICH. (A) Immunofluorescence staining for VIM (purple) and GFAP (green) in ICH mouse brain at 3d post-PBS transplantation. Bar = 100μm. GFAP and VIM were expressed in reactive astrocytes around the lesion area. (B) Immunofluorescence staining for VIM (purple) and GFAP (green) in the ICH mouse brain at 3d post-BM-MSCs transplantation. Bar = 100μm. After the transplantation of BM-MSCs, VIM was strongly expressed, whereas GFAP remained at the same level. (C) The results of relative fluorescence intensity of GFAP and VIM, plotting into a histogram of five randomly fields. (DI) Western blotting analysis of GFAP, VIM, ALDH1L1, EAAT1, p-MST1, MST1, p-YAP and YAP expression in the ICH mouse brain of Sham, PBS, and BM-MSCs treatment at 3d. Expressions were normalized against the internal reference GAPDH. The fold change values were calculated by normalizing to the sham group. The results were plotted as mean ± SD (n = 6). *p < 0.05, compared with sham group.