Figure 3. BM-MSCs coculture protected astrocytes from neurotoxicity induced by hemin. (A) Astrocytes were exposed to 0, 5, 10, 20, 30, 40, 50μM hemin for 24 h with or without BM-MSCs coculture, then the cell viability was evaluated by CCK-8. (B) Astrocytes were exposed to 30μM hemin with or without BM-MSCs for 24 h, and the cell death was evaluated by LDH releasing assay; (C) mRNA expression of TNFα, IL-6, and IL-10 was checked. (D) mRNA expression of TEAD2 was checked. (E, F) Ki67 staining (green) was applied to mark cell proliferation, bar = 25μm. The results plotted as mean ± SD (n = 4), and the relative expression of the mRNA and results of proliferation rate were normalized to control and plotted into a histogram. *p< 0.05, **p < 0.01, ***p < 0.001 compared with ctrl; #p < 0.001.