Figure 4. BM-MSCs coculture induced an epithelial-mesenchymal switching in astrocytes. (A) Immunofluorescence staining of astrocytes for VIM (red) and GFAP (green). The cell nuclei were counterstained with DAPI (blue). Bar = 25 μm. (B) The results of relative fluorescence intensity of GFAP and VIM, plotting into a histogram of five randomly fields. (C–F) Western blotting analysis of GFAP, VIM, ALDH1L1, and EAAT1 in astrocytes exposure to 30 μM hemin, with or without BM-MSCs coculture for 24 h. BM-MSCs coculture increased VIM, GFAP and EAAT1 expression, while decreasing ALDH1L1 expression. Expressions were normalized against the internal reference GAPDH. The fold change values were calculated by normalizing to control samples. The results were plotted as mean ± SD (n = 6). *p < 0.05, **p < 0.01 compared with ctrl group.