Research Paper Volume 12, Issue 7 pp 6306—6323

Bone marrow mesenchymal stem cells transplantation alleviates brain injury after intracerebral hemorrhage in mice through the Hippo signaling pathway

Figure 5. BM-MSCs coculture promoted YAP nuclear translocation in astrocytes. (A) Immunofluorescence staining of astrocytes with YAP (green). The cell nuclei were counterstained with DAPI (blue). BM-MSCs coculture treatment triggered YAP nuclear translocation in astrocytes exposure to 30 μM hemin. Bar = 25 μm. (BD) Western blotting analysis of p-MST1, MST1, p-YAP and YAP expression in astrocytes exposure to 30 μM hemin, with or without BM-MSCs coculture for 24 h. (E, F) Western blotting analysis of cytoplasmic and nucleus extraction samples from astrocytes, with or without BM-MSCs coculture, with YAP antibody. GAPDH and H3 were used as a loading control for cytoplasmic and nucleus protein, respectively. The histogram showing the results of densitometric analysis of nucleus/cytoplasmic YAP expression in astrocytes cocultured with BM-MSCs or not. The results were normalized to control and plotted as mean ± SD (n = 4). *p < 0.05, **p < 0.01, compared with ctrl group.