Research Paper Volume 12, Issue 7 pp 6306—6323

Bone marrow mesenchymal stem cells transplantation alleviates brain injury after intracerebral hemorrhage in mice through the Hippo signaling pathway

Figure 6. si-YAP counteracted BM-MSCs coculture induced cell proliferation and GFAP/VIM switching. (A) Western blotting analysis of YAP expression in ctrl, si-NC, and si-Nrf2 transfected astrocytes. (B) The results of densitometric analysis of the bands were plotted as mean ± SD (n = 4). Over 83% of YAP expression was suppressed by si-YAP. ***p < 0.001 compared with si-NC group. (C) Representative pictures of Ki67 immunofluorescence staining. Ki67-positive cells were labeled in green. Bar = 50μm. (D) The results of the proliferation rate and densitometric analysis of the bands were plotted into a histogram of five randomly fields. (E). mRNA expression of TNFα, IL-6, and IL-10 was checked. (F) mRNA expression of TEAD2 was checked. (G, H) Western blotting analysis of p-MST1, MST1, GFAP, VIM, ALDH1L1, and EAAT1 protein expression was examined. The relative expression was normalized to control. The results of densitometric analysis of the bands were plotted as mean ± SD (n = 4). *p < 0.05, **p < 0.01.