Figure 5. ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. (A) Analysis of BDNF, TrkB, p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. (B–C) In EP and EP-sham-DBS groups, BDNF and TrkB levels were elevated compared with the control group. With ANT-DBS, less BDNF and TrkB were observed. (n=3 in each group) (D–F) In the epileptic animal model, the ratio of p-Akt/Akt, p-ERK/ERK and p-S6K/S6K was enhanced in the hippocampus, and reversed by ANT-DBS. (n=3 in each group) *P < 0.05; **P < 0.01; ***P < 0.001; NS, P > 0.05. Data were presented as mean ± SD. I, control group; II, EP group; III, EP-sham-DBS group; IV, EP-DBS group.