Research Paper Volume 12, Issue 7 pp 6352—6369

FAM46C inhibits cell proliferation and cell cycle progression and promotes apoptosis through PTEN/AKT signaling pathway and is associated with chemosensitivity in prostate cancer

Figure 5. FAM46C expression was associated with cell cycle, apoptosis and PTEN signaling pathways. (A) GSEA analysis in patients with prostate cancer with higher versus lower FAM46C expression based on TCGA database showed that cell cycle, apoptosis and PTEN pathways were correlated with FAM46C expression. NES: normalized enrichment score. After 22RV1 (B) and DU145 cells (C) were transduced with pLKO.1-shFAM46C#1 and pLKO.1-shFAM46C#3, while DU145 cells (D) were transduced with pLVX-Puro-FAM46C, the expression of PTEN, P27, cleaved Caspase-3, AKT and p-AKT was measured by western blot. ***P<0.001 compared with pLKO.1-shNC or Vector.