**Figure 1.** **Stable transfection-induced PANC-1 cell senescence.** (**A**) Morphology graph of vector PANC-1 cells. (**B**) Confocal fluorescent graph of the nuclei (blue fluorescence) morphology of vector PANC-1 cells. (**C**) An increased percentage of vector PANC-1 cells was arrested in G2/M phase. Graphic (top) and percentage (bottom) representations of cell cycle distributions are shown. This experiment was repeated independently three times. (**D**) Decreased BrdU incorporation during DNA synthesis in vector PANC-1 cells. Data are presented as the mean ± S.E.M, n = 4 (***p* < 0.01). (**E**) Cell growth curve shows decreased proliferation of vector PANC-1 cells. Data are presented as the mean ± S.E.M, n = 3 (**p* < 0.05, ***p* < 0.01, ****p* < 0.001). (**F**) Decreased ability of vector PANC-1 cells to form colonies when seeded at the indicated dilutions. (**G**) Quantitative RT-PCR analysis of the upregulated key SASP factor, *IL-8* mRNA, in vector PANC-1 cells. Data are presented as the mean ± S.E.M, n = 3 (****p* < 0.001). (**H**) SA-β-gal staining and positive senescence signal of vector PANC-1 cells. This experiment was repeated independently three times. (**I**) Activation of extrinsic apoptosis pathways was analyzed. See also Supplementary Figures 1 and 2.