Research Paper Volume 12, Issue 8 pp 6733—6755

Carnitine palmitoyltransferase 1C contributes to progressive cellular senescence

Figure 5. Malignance is reduced in low-CPT1C-expressing senescent vector PANC-1 cells. Sensitivity to metabolic stress from (A) glucose deprivation (0.5 mM glucose) and (B) glycolytic inhibition (20 mM 2-deoxyglucose) of senescent vector PANC-1 cells at the indicated time points. Data are presented as the mean ± S.E.M, n = 5 (*p < 0.05, **p < 0.01, ***p < 0.001). (C) Sensitivity to metabolic stress from rapamycin stimuli of senescent vector PANC-1 cells at the indicated concentrations. Data are presented as the mean ± S.E.M, n = 5 (**p < 0.01, ***p < 0.001). (D) Transwell migration and Matrigel invasion capacities of senescent vector PANC-1 cells. Data are presented as the mean ± S.E.M, n = 3 (*p < 0.05, **p < 0.01). (E) Tumor sizes are presented as the mean ± S.E.M over time, (n = 5) (**p < 0.01, ***p < 0.001). (F) Images of tumors after excision on day 42 post-implantation. (G) Comparison of dissected tumor weights (mean ± S.E.M, ***p < 0.001). See also Supplementary Figure 5.