Research Paper Volume 12, Issue 7 pp 6385—6400

Downregulation of LncRNA NORAD promotes Ox-LDL-induced vascular endothelial cell injury and atherosclerosis

Figure 5. NORAD-knockdown aggravates the abnormal serum lipid condition and increased the proinflammatory molecule expression induced by HFD in ApoE−/− mice. ApoE−/− mice were given Ad-NORAD or control Ad-EGFP injection after 8 weeks of HFD, and HFD was maintained for another 8 weeks. C57BL/6 J mice fed with the normal diet were set as the WT control group. (A) NORAD levels in the aortic sinus were analyzed through qRT-PCR (n = 3, *P < 0.05 vs. Ad-EGFP). Serum levels of lipids, TC (B), TG (C), and LDL-C (D) were detected. Values were shown as mean ± SD (n = 8). *P < 0.05, **P < 0.01 vs. WT group, and ##P < 0.01 vs. Ad-EGFP group. (E) Western blot was used to detect the ICAM, VCAM, and IL-8 expression levels (n = 8, *P < 0.05 vs. WT group, and #P < 0.05 vs. Ad-EGFP group). The mRNA levels of ICAM (F), VCAM (G), and IL-8 (H) in thoracic aortas were detected through qRT-PCR (n = 8, *P < 0.05 vs. WT group, and #P < 0.05 vs. Ad-EGFP group).