Figure 6. NORAD-knockdown promotes atherosclerosis development in ApoE−/− mice. ApoE−/− mice were given Ad-NORAD or control Ad-EGFP injection after 8 weeks of HFD, and the HFD was maintained for another 8 weeks. C57BL/6 J mice fed with the normal diet were used as the blank WT control group. (A) Representative micrographs and quantification of lesion area in the aortic sinus observed through H&E staining. (B) Representative micrographs and quantification of the collagen fibers area in the aortic sinus observed through Masson staining. (C) Representative en face images of the entire aorta with Oil Red O staining. The atherosclerotic lesion areas were stained red. Oil Red O-positive areas were analyzed with ImageJ. Data were shown as mean ± SD, n = 8. **P < 0.01 vs. WT group, *P < 0.05 vs. WT group, and #P < 0.05 vs. Ad-EGFP group.