Figure 2. circTNFRSF21 inhibits TNFRSF21 expression. (A) siRNA targeting the junction of the covalently joined 3' and 5' ends of circ_TNFRSF21 was shown. F1/R1 and F2/R2 were used to amplify TNFRSF21 mRNA. (B) Detection of circ_TNFRSF21 expression in HEC1-A after silencing circ_TNFRSF21. TNFRSF21 mRNA (C) and protein level (D) after silencing circ_TNFRSF21 in HEC1-A. Overexpression of circ_TNFRSF21 (E) significantly decreased TNFRSF21 transcription (F) and protein synthesis (G). *P<0.05, **P<0.01, ***P<0.001. Data are represented as mean +/- SEM.