Figure 6. miR-1227 targets MAPK13 and inhibits EC cell proliferation. (A) Predicted MAPK13 3’UTR-miR-1227 interaction. Detection of MAPK13 mRNA transcription (B) and protein level (C) after overexpressing miR-1227 mimic in HEC1-A and Ishikawa cells. (D) Luciferase assay detects WT or miR-1227 binding sites mutated MAPK13 3’UTR activity in the presence or absence of miR-1227. (E) Western blot detects MAPK13 expression in EC cell lines and HEuEC. (F) Western blot results of MAPK13 after knocking down MAPK13 in HEC1-A and Ishikawa cells. GAPDH was used as internal control. (G) CellTiter-Glo detect cell viability after knocking down MAPK13 in HEC1-A and Ishikawa cells, cell viability was tested 5 days after transduction. *P<0.05, **P<0.01, ***P<0.001. Data are represented as mean +/- SEM.