Research Paper Volume 12, Issue 7 pp 6415—6435

Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation

Figure 7. ES induces Sirt3-mediated deacetylation of ATG5 in VbP-treated macrophages. (A) Representative western blots of ATG5 expression following immunoprecipitation with an anti-Sirt3 antibody (top), Sirt3 expression following immunoprecipitation with an anti-ATG5 antibody (middle) and ATG5 and Sirt3 expression in total cell lysates (bottom). (B) Representative western blot and quantitative analysis of acetylated-ATG5 in macrophages treated with VbP, 3-TYP, and ES. n =3; ***P<0.001 vs. control cells; #P<0.05 and ###P<0.001 vs. VbP-treated cells.