Research Paper Volume 12, Issue 8 pp 6793—6807

LINC00514 drives osteosarcoma progression through sponging microRNA-708 and consequently increases URGCP expression

Figure 2. Effects of long intergenic nonprotein-coding RNA 00514 (LINC00514) knockdown on the proliferation, colony formation, apoptosis, cell cycle, migration, and invasion of osteosarcoma (OS) cells. (A) LINC00514 expression in OS cell lines (HOS, MG-63, U2OS, and SAOS-2) and a normal human osteoblasts cell line (hFOB 1.19) was determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR). (B) HOS and MG-63 cells were transfected with si-LINC00514 and si-NC. LINC00514 silencing was verified by RT-qPCR. (C) Cell Counting Kit-8 assay was carried out to determine the proliferation of HOS and MG63 cells after LINC00514 was knocked down. (D) Colony formation assay presented that the colony-forming ability was impaired in HOS and MG63 cells after LINC00514 knockdown. (E, F) The apoptosis rate and cell cycle status of HOS and MG63 cells with LINC00514 silencing was tested via flow cytometry analysis. (G, H) Representative images revealing the transwell migration and invasion assays used to examine the impacts of LINC00514 underexpression on HOS and MG-63 cell migration and invasion. *P < 0.05 and **P < 0.01.