Research Paper Volume 12, Issue 8 pp 6793—6807

LINC00514 drives osteosarcoma progression through sponging microRNA-708 and consequently increases URGCP expression

Figure 4. URGCP promotes the malignant phenotype of OS cells in vitro. (A) Western blotting confirmed URGCP silencing in HOS and MG-63 cells after transfection with si-URGCP. (B, C) Proliferation and colony formation of URGCP-silenced HOS and MG-63 cells were determined by Cell Counting Kit-8 and colony formation assays, respectively. (D, E) Flow cytometry analysis was performed to evaluate the effect of URGCP depletion on HOS and MG-63 cell apoptosis and cell cycle distribution. (F, G) Migrated and invasive URGCP-deficient HOS and MG-63 cells were quantified using transwell migration and invasion assays. *P < 0.05 and **P < 0.01.